Fig. 3.

Boxplot of coefficients of variation for all cDNA-assay combinations over all time points of both quantitative PCR techniques. We observed an inverse correlation between the CV and the relative abundance of each isoform; CVs are consistently higher for isoforms with lower expression. CVs for each transcript range within the same order of magnitude and all experiments show very small standard errors, leading us to conclude that precision, reproducibility and repeatability are not an issue with either quantitative PCR technique.