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. 2017 Oct 10;15:71. doi: 10.1186/s12951-017-0301-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Quantification of Eu fluorescence. Eu fluorescence signal intensity increased linearly with increasing Eu concentration with both enhancer solutions. Therefore, both systems are suitable for quantitative detection of Eu³⁺ in solutions with neutral pH (n = 5). Regression analysis for HEE (r² = 0.9999) and for DELFIA^® (r² = 0.9994)