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. 2017 Oct 10;15:71. doi: 10.1186/s12951-017-0301-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Stability of Eu fluorescence in presence of free iron(III) ions. The fluorescence intensity developed by each enhancer solution was set to 100% for a solution with constant c_final(Eu) = 50 nmol/L and without iron(III). The fluorescence signal intensity developed by DELFIA was reduced to 10% when exposed to c(Fe) > 1 µmol/L while the Eu fluorescence intensity developed with HEE remained at 100% in the presence of c(Fe) up to 100 µmol/L. The SD of the mean fluorescence signal (n = 3) for HEE variates between 0.7 and 8% and between 0.3 and 11% for DELFIA^Ⓡ