Table 1.

Primers used for genotyping

Polymorphism	Primers	Annealing temp (°C)	Restriction enzyme	Band sizes (bp)
TNF -238G > A	F: 5′-AAA CAG ACC ACA GAC CTG GTC-3′	59	BamHI	G: 130, 20
	R: 5′-CTC ACA CTC CCC ATC CTC CCG GAT C-3′			A: 150
TNF -308G > A	F: 5′-GAG GCA ATA GGT TTT GAG GGC CAT-3′	58	NcoI	G: 87, 20
	R: 5′-GGG ACA CAC AAG CAT CAA G-3’			A: 107
IL1B -511 T > C	F: 5′-TGG CAT TGA TCT GGT TCA TC-3’	58	AvaI	T: 305
	R: 5′-GTT TAG GAA TCT TCC CAC TT-3’			C: 190, 115
IL1B 3954C > T	F: 5′-GTT GTC ATC AGA CTT TGA CC-3’	54	TaqI	T: 250
	R: 5′-TTC AGT TCA TAT GGA CCA GA-3’			C: 136, 114
IL6 -174G > C	F: 5′-GGA GTC ACA CAC TCC ACC T-3’	59	SfaNI	G: 532
	R: 5′-GTG GGG CTG ATT GGA AAC C-3’			C: 474, 58
IL6 -634C > G	F: 5′-GAG AGG CCT TGA AGT AAC TG-3’	58	BsrBI	C: 180
	R: 5′-AAC CAA AGA TGT TCT GAA CTG A-3’			G: 120, 60
IL10 -1082A > G	F: 5′-GTA AGC TTC TGT GGC TGG AGT-3’	60	MnlI	A: 130
	R: 5′-TTT CCA GAT ATC TGA AGA AGT CCT G-3’			G: 100, 30
IFNG 874A > T	F: 5′-TCA ACA AAG CTG ATA CTC CA-3’	61	–	261
	R1: 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3’
	R2: 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3’