(a) and (b) Diurnal gene expression analysis of CO and FT in Col-0, cdfq, and SUC2:HA-tpl-1 plants. Experiments were performed on 14-day-old seedlings grown in long days, with 3-hour resolution. Means +/− SEM were calculated from four independent experiments. (c and d) Gene expression analysis of CO (c) and FT (d) in Col-0, cdfq, and SUC2:HA-tpl-1 plants in short days. Experiments were performed on 14-day-old seedlings in short days, with 6-hour resolution. Means +/- SEM were calculated from four independent experiments. (e and f) Gene expression analysis of CO (e) and FT (f) in Col-0, cdfq, and SUC2:HA-tpl-1 plants over developmental time. Plants were harvested over time from 5-days to 19-days old at the ZT13 time point of long days. Means +/− SEM were calculated from four independent experiments. Arrowheads indicate the day at which either cdfq or SUC2:HA-tpl-1 plants were first observed having a single plant begin flowering (the wild-type plants did not start flowering during the experiment).