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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Plant J. 2017 Sep 5;92(2):244–262. doi: 10.1111/tpj.13649

Figure 7. Introduction of SUC2:HA-tpl-1 into co and ft mutants caused earlier flowering, and TPL directly regulates TSF expression in long days.

Figure 7

(a) Representative images of SUC2:HA-tpl-1/co-101 and SUC2:HA-tpl-1/ft-101 plants and their parental genetic backgrounds (co-101 and ft-101) under long-day and short-day photoperiods at flowering. Scale bars=2 cm. (b and c) Quantification of flowering time by total leaf number at bolting from (a) under long days (b) and short days (c). Means +/− SEM were calculated from N=16 individuals. *P < 0.05,**P < 0.01 (one-tailed t test). (d) Diurnal gene expression analysis of TSF in Col-0, cdfq, and SUC2:HA-tpl-1 plants in long days. Experiments were performed on 14-day-old seedlings grown in long days, with 3-hour resolution. Means +/− SEM were calculated from four independent experiments. (e) ChIP experiment for TPL binding to the TSF genomic locus at ZT12, in Col-0, TPL:TPL:3HA, and TPL:TPL:3HA/35S:CDF1. Means and +/− SEM were calculated from four independent experiments. **P < 0.01 (one-tailed t test). Plants were 2 weeks old and grown under long-day photoperiods. (f) Model for CDF1-TPL dependent regulation of CO and FT in the morning and the evening.