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. 2017 Oct 10;7:12916. doi: 10.1038/s41598-017-13365-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protective effect of sodium fluorocitrate is specific for palmitate-induced lipotoxicity. INS-1 cells were treated with 0.2 mM sodium fluorocitrate (SFC) in the presence of (a) 50 μM H₂O₂ for 3 h, (b) 1 μM thapsigargin (TG) for 9 h, (c) 30 mM streptozotocin (STZ) for 12 h or (d) cytokine mixture (CYT; 5 ng/ml of IL-1β, 10 ng/ml of TNF-α, 50 ng/ml of INF-γ) for 24 h. Cell death was measured with Cell Death Detection ELISA kit. Data are presented as mean ± SE and analyzed by two-way ANOVA. ^### p < 0.001 vs. PBS-teated INS-1 cells. NS: not significant.