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. 2017 Oct 10;7:12911. doi: 10.1038/s41598-017-11756-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lipofuscin staining with Fontana-Masson in liver from the NF and OF groups at PND 24 and 7 months of life. Lipofuscin staining was evaluated with Fontana-Masson at PND 24 in the NF (A) and OF (E) groups (20x) and at 7 months of life in the NF (C) and OF (G) groups (40x). A “stack image” was generated from NF (B) and OF (F) groups at PND 24 and from NF (D) and OF (H) groups at 7 months of life to quantify the stained structure area using ImageJ. The results are reported as the percentage of lipofuscin staining area of the total area. ***p < 0.001. These images are representative from n = 8 animals/group.