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. 2017 Oct 10;7:12911. doi: 10.1038/s41598-017-11756-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hepatic fibrosis in the NF and OF groups at 7 months of life. Hepatic fibrosis was evaluated with Masson’s Trichrome at 10x in the NF (A-B) and OF (A-C) groups. A “stack image” was generated from NF (A-B) and OF (A-C) groups, respectively (A-A) and (A-D), to quantify the fibrotic area using ImageJ. A specific zone was enlarged at 20x in the NF (A-E) and OF (A-F) groups. ***p < 0.001. These images are representative from n = 8 animals/group. Alpha-SMA (α-SMA) protein expression was measured by western blot in the NF (white) and OF (gray) groups (B). Cropped blots are displayed. Representative images are presented and full-length western blots are presented in supplemental data 1. The values are reported as the mean ± SD; *p < 0.05; n = 7 animals/group.