Table 1. Primers of target genes.

Gene	Accession number	Forward (5′–3′)	Reverse (5′–3′)
GAPDH	NM_001206359.1	ACCTCCACTACATGGTCTACA	ATGACAAGCTTCCCGTTCTC
Aggrecan	NM_001164652.1	CGTGGTCCAGCACTTCTAAA	AGTCCACTGAGATCCTCTACTC
Collagen II	XM_001925959.4	CCGGGTGAACGTGGAGAGACTG	CGCCCCCACAGTGCCCTC
ADAMTs-4	XM_003481414.2	TTCAACGCCACGTTCTACTC	GCCGGGATGATGAGGTTATTT
MMP-3	NM_001166308.1	GCCCGTTGAGCCCACAGAATCTAC	GGAAGAGGTGGCCAAAATGAAGAG
TIMP-1	NM_213857.1	CCTGACATCCGGTTCATCTA	CAGTTGTCCAGCTATGAGAAAC
TIMP-3	XM_003126073.4	GGATTGTGTAACTTTGTGGAGAG	GGCAGGTAGTAGCAGGATTTA
P16	XM_013993499.1	TGCAGTTGCCTACCTCTGAA	CGAATCCGCACAGTAATCAA
P53	NM_213824.3	CCTTAAGATCCGTGGGCGT	GCTAGCAGTTTGGGCTTTCC
P21	XM_013977858.2	CCTCTAAGGTTGGGCAGGGT	TTTCACTGGACAGAGGGGCT