Inflammatory cell death of APCs does not impact NF-κB and MAPK signaling. (a,b) DCs were infected with ST-OVA (10 MOI) and western blot analysis was performed on cell lysates collected at various time intervals. (c) DCs from WT, RipK3−/minus;, Caspase-1,11−/minus; and Caspase-1,11−/minus;RipK3−/minus; mice were infected with ST (1 MOI) for 30 min followed by treatment with gentamycin to eliminate extracellular bacteria. Expression of cytokines and chemokines was measured in the supernatants collected at 24 h supernatants post-infection. The data are shown as mean ±SEM and is representative of 2–3 separate experiments. Statistical significance was determined by unpaired student t-test (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001)