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Generation of MUC1-expressing and mutant cells. (a) Schematic diagram of MUC1 transfectants. MUC1 expression in the transfectants of human colon cancer HCT116 (b), the human breast epithelial HBL-100 (c) cells were analysed by immunoblotting with anti-MUC1 antibodies against the MUC1 extracellular domain (B27.27) and intracellular domain (CT2). The blots were also probed with anti-actin antibody for protein loading
