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. 2017 Aug 4;24(11):1963–1974. doi: 10.1038/cdd.2017.123

Figure 3.

Figure 3

Low concentration of ABT-737 protects hippocampal neurons from glutamate-induced death. (a-d) Primary hippocampal neurons were treated with ABT-737 (10 nM), glutamate (20 μM) or a combination of both for 24 h. (a) PI and calcein-stained neurons. Green: calcein; red: PI. Scale bar=20 μm. (b) Number of PI-positive cells (n=3 independent cultures). (c) Calcein retention (n=4 independent cultures). (d) LDH release (n=9 independently treated plates from three cultures). (e) Primary hippocampal neurons were treated with WEHI-539 (10 nM), glutamate (20 μM) or a combination of both for 24 h. Bar graphs show LDH release (n=3 independent cultures). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001, one-way ANOVA