Figure 4.

Low concentration of ABT-737 augments mitochondrial TMRM uptake. Primary hippocampal neurons were treated with ABT-737 (10 nM), glutamate (20 μM) or a combination of both. TMRM (5 nM) was added into cell the culture medium at 6 h of incubation. (a) TMRM stained neurons. Scale bar=10 μm. (b) TMRM intensity (n=10 independently treated experiments from four cultures). (c) ATP level (n=3 independent cultures) at 8 h. (d) Primary hippocampal neurons were treated with WEHI-539 (10 nM), glutamate (20 μM) or a combination of both for 8 h. Bar graph shows ATP level (n=4 independent cultures). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001, one-way ANOVA