Figure 8.

Depletion of ATP synthase c-subunit protects neurons and neurites against ΔN-BcL-xL-aggravated glutamate-induced neuronal death. Primary hippocampal neurons expressed scrambled GFP-labeled shRNA, GFP-labeled ATP c-subunit shRNA and empty vector or ΔN-Bcl-xL with/without glutamate treatment. (a and b) Images and group data of PI staining of cultured hippocampal neurons at 24 h after glutamate treatment (n=3 independent cultures per group). (c) Number of neurites attached to soma (n=42-55 micrographs) *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001, one-way ANOVA