. 2017 Aug 25;24(11):1845–1852. doi: 10.1038/cdd.2017.134

Table 2. Some key factors associated with the development of mouse oocyte and follicle in vitro.

Factor/s	Culture method	References
FSH	Essential for follicular survival and development, contributes to granulosa cells proliferation	^{44, 47, 48, 49, 50, 51}
EGF and IGF-I	Have an effect on follicle growth and development and embryo development after fertilization.	^{11, 52, 53}
Activins	Promote antral cavity formation and granulosa cells proliferation, affect follicle growth and development	^{35, 54, 55, 56, 57, 58, 59, 60}
KL (or SCF)	Promote the oocyte survival and growth	²¹
cAMP	Control of meiotic progression, gap–junctional communication between granulosa cells and oocyte and antral cavity formation	^{61, 62, 63, 64, 65, 66}
PVP	Maintains the integrity of follicles or COCs in culture	^{67, 68}
Ascorbic acid	Acts likely as antioxidant preventing deleterious action of the free radicals	^{69, 70}
Collagen	Favours follicle/COC adhesion, stimulates cell proliferation and development and support biological signalling pathways	^{3, 11, 42, 43}
ICI182780	Antagonist of oestrogen receptor, its addition to the culture medium of ovarian tissue results in a marked increase of secondary follicle number	^{2, 3}
Theca layers	Their removal exposes COC to the culture medium resulting in considerable improvement of its development and the acquisition of meiotic maturation by the oocyte	^{2, 3}