Figure 1.

Loss of BIM augments resistance of Atm^−/− thymocytes to DNA damage-induced apoptosis, but does not impact lymphoma development. (a) Thymocytes survival was determined following treatment with the indicated agents relative to untreated cells at 24 h. n=3–9. (b) Mice were reconstituted with HSPCs and lymphoma-free survival was determined (median survival: Atm^−/−Bim^−/− 232d, Atm^−/−Bim^+/− 276d and Atm^−/−Bim^+/+ 251d). Thymus weight from the lymphoma-bearing animals and tumour immunophenotype was determined (donor origin was confirmed with congenic CD45 staining). n=8–22; tumour type mean shown. (a) *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; black (wild-type vs Bim^−/−), blue (wild-type vs Atm^−/−), yellow (Atm^−/− vs Atm^−/−Bim^+/−) and red (Atm^−/− vs Atm^−/−Bim^−/−). Mean±S.E.M. depicted