Fig. 2.

EGCG improves ROS homeostasis that is crucial for C. elegans healthspan extension. (A) EGCG induced a transient ROS formation early and exhibited lower ROS levels later in life, 2 h 5 mM paraquat treatment (PQ) was the positive control. (B) The antioxidant NAC or BHA abolished the lifespan-extending effect of EGCG. (C) The mix of EGCG and PQ (200 μM) achieved a robust increase in lifespan, substantially greater than those observed in any experiment with a single compound. (D) EGCG increased 5 mM PQ resistance following 6 d treatment. (E) EGCG attenuated age-associated changes in antioxidative genes. Heat map depicting log2 changes in gene expression for antioxidative genes elicited by EGCG. EGCG was added on day 0 of adulthood and RNA samples were harvested on Day 0 (Vehicle treatment), Day 2, Day 6, and Day 10. (F) EGCG increased SOD and CAT activities after 6 days treatment. Bar graphs are expressed as mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001.