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. 2017 Sep 29;14:305–315. doi: 10.1016/j.redox.2017.09.019

Fig. 4.

Fig. 4

EGCG-induced longevity requires AAK-2, the C.elegans AMPK homolog, and SIR-2.1, the C.elegans SIRT1 homolog. (A) EGCG did not extend lifespan in the aak-2(ok524) mutant. (B) Total ROS level was increased by EGCG treatment and by aak-2(ok524) mutations for 2 days. (C) EGCG did not up-regulate antioxidative gene expression in the aak-2(ok524) mutant compare to age-matched Vehicle-treated worms. (D) EGCG treatment for 6 d did not increase resistance to oxidative stress in the aak-2(ok524) mutant. (E) AAK-2/AMPK was required for EGCG-induced mitochondrial biogenesis. (F) Aging decreased worm NAD+ level in Vehicle- and EGCG-treated worms, however, EGCG supplementation increased NAD+ level. PQ (5 mM) treatment was the negative control. (G) EGCG did not extend lifespan in the sir-2.1(ok434) mutant. (H) EGCG increased NAD+ level in the sir-2.1(ok434) mutant, not aak-2(ok524) or aak-2(ok524), sir-2.1(ok434) mutant. (I) EGCG did not increase lifespan of the aak-2(ok524), sir-2.1(ok434) mutant. Bar graphs are expressed as mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001.