Table 3.
Results of RT-qPCR analysis reporting the % inhibition of IL-8 mRNA in TNF-α-induced IB3-1 cells cultured with 1 μM and 10 μM concentrations of TMA derivatives.
| Derivative | 1 μM (% inhibition) | 10 μM (% inhibition) | Inhibition of NF-κB/DNA complex (MIC) μM |
|---|---|---|---|
| 1 | 57 | 56 | <12.5 (∗) |
| 5 | 33 | 31 | 12.5–25 |
| 8 | 51 | 52 | 25–50 |
| 12 | 0 | na | 12.5 |
| 16 | 18 | 21 | 25–50 |
| 23 | 3 | 11 | 2–505 |
| 25 | na | na | 50–100 |
| 26 | 55 | 83 | 50–100 |
| 27 | 16 | na | <12.5 (∗) |
| 28 | 21 | 36 | 12.5–25 |
| 29 | na | 12 | <12.5 (∗) |
| 30 | 10 | na | 12.5–25 |
| 31 | na | na | 12.5 |
| 32 | 73 | 52 | <12.5 (∗) |
| 33 | 68 | 67 | 12.5–25 |
| 34 | 66 | 47 | <12.5 (∗) |
| 35 | 56 | 37 | 25–50 |
| 36 | 77 | 74 | 12.5–25 |
| 37 | na | na | 12.5–25 |
| 38 | 36 | 81 | 25 |
na: not active at the used concentrations; MIC: minimal inhibitory concentration. ∗In the EMSA experiments, 12.5 μM was the minor concentration used.