Fig. 3.

Synthetic growth and morphology defects between SSE1 and SBF components. a Wild type (BY4741), and the indicated isogenic derivative strains (see Table 1) were serially diluted, plated on YPD and incubated at 30 and 37°C for 2 days. A parallel dilution series was subject to the alkaline phosphatase cell leakage assay after 2 days of growth at 37°C as described in “Materials and methods,” and a representative dilution spot is shown for all strains (BCIP). b The same strains were analyzed for morphology using Normarski microscopy. Representative micrographs are shown. c Quantitation of the morphological types observed in (b). Populations of at least 100 cells were categorized as normal (white bars), elongated (gray bars), or amorphous (black bars) as described in “Materials and methods”