Table 3. Allele-specific gene expression of relevant genes in hybrid strains W27 and VIN7 in Tempranillo grape must fermentation.
Sc: S. cerevisiae; Sk: S. kudriavzevii. Sc/Sk is the relative expression of Sc alleles divided by relative expression of Sk. nd: not determined; <dl: below detection limit. C: S. cerevisiae allele; K: S. kudriavzevii allele. All values were normalized relative to the W27 LSM8 S. cerevisiae allele. *: Data obtained from previous work 6, 11, 12.
| Hybrid strain | Gene | Genomic
Composition* |
Relative expression of allele | Ratio
(Sc/Sk) |
Total gene
transcription levels |
|
|---|---|---|---|---|---|---|
| S. cerevisiae | S. kudriavzevii | |||||
| W27 | NUG1 | CKK | 299.01±19.21 | 40.30±3.65 | 7.42 | 339.31±19.55 |
| PDC5 | CK | 380.20±53.86 | 41.19±4.48 | 9.23 | 421.39±54.04 | |
| LSM8 | CK | 1.00±0.12 | 6.58±0.16 | 0.15 | 7.58±0.19 | |
| NSR1 | CK | 1275.25±102.97 | 186.14±12.48 | 6.85 | 1461.39±103.73 | |
| GPD1 | CC | 895.05±19.11 | <dl | - | 895.05±19.11 | |
| GUT2 | CC | nd | <dl | - | <dl | |
| VIN7 | NUG1 | CCK | 392.08±14.75 | 65.54±2.20 | 5.98 | 457.62±14.91 |
| PDC5 | CCK | 296.04±22.38 | 16.24±6.53 | 18.23 | 312.28±23.31 | |
| LSM8 | CCK | 0.62±0.07 | 4.11±0.40 | 0.15 | 4.73±0.41 | |
| NSR1 | CCC | 895.05±19.11 | <dl | - | 895.05±19.11 | |
| GPD1 | CCK | 648.51±14.16 | 1850.50±57.72 | 0.35 | 2499.01±59.43 | |
| GUT2 | CCK | 113.86±13.86 | 81.98±1.84 | 1.39 | 195.84±13.98 | |