Table 2.
Graph | Data structure | Type of test | Dataset | p value |
---|---|---|---|---|
Fig. 1A | Nonparametric | Mann-Whitney test | AMPAR-EPSC | 0.97 |
NMDAR-EPSC | 0.51 | |||
AMPAR/NMDAR ratio | 0.97 | |||
Fig. 1B | Nonparametric | Mann-Whitney test | AMPAR-EPSC | 0.69 |
NMDAR-EPSC | 0.47 | |||
A/N ratio | 0.33 | |||
Fig. 1C | Nonparametric | Mann-Whitney test | AMPAR-EPSC | 0.79 |
NMDAR-EPSC | 0.79 | |||
A/N ratio | 0.47 | |||
Fig. 1D | Parametric | Student’s t test | PPR of pSup | 0.86 |
PPR of shScr | 0.48 | |||
PPR of shLuc | 0.76 | |||
Fig. 2A | Nonparametric | Mann-Whitney test | GABAAR-IPSC | 0.21 |
AMPAR-EPSC | 1 | |||
AMPAR/GABAAR ratio | 0.55 | |||
Fig. 2B | Nonparametric | Mann-Whitney test | GABAAR-IPSC | 0.43 |
AMPAR-EPSC | 0.41 | |||
A/G ratio | 0.56 | |||
Fig. 2C | Nonparametric | Mann-Whitney test | GABAAR-IPSC | 0.69 |
AMPAR-EPSC | 0.92 | |||
A/G ratio | 0.89 | |||
Fig. 2D | Nonparametric | Mann-Whitney test | GABAAR-IPSC | 0.15 |
AMPAR-EPSC | 0.24 | |||
A/G ratio | 0.58 | |||
Fig. 2E | Parametric | Student’s t test | PPR of pSup | 0.63 |
PPR of shScr | 0.74 | |||
PPR of shLuc | 0.31 | |||
Fig. 3B | Parametric | One-way ANOVA post hoc Tukey | Spine density: pSup vs shLuc | 0.45 |
pSup vs shScr | 0.09 | |||
shScr vs shLuc | 0.65 | |||
Spine length: pSup vs shLuc | 0.99 | |||
pSup vs shScr | 0.68 | |||
shScr vs shLuc | 0.63 | |||
Spine width: pSup vs shLuc | 0.96 | |||
pSup vs shScr | 0.37 | |||
shScr vs shLuc | 0.53 | |||
Fig. 4A | Parametric | Two-way ANOVA post hoc Tukey | −100 pA | |
untrans vs shLuc | >0.9999 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | >0.9999 | |||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
−50 pA | ||||
untrans vs shLuc | >0.9999 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | >0.9999 | |||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
0 pA | ||||
untrans vs shLuc | >0.9999 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | >0.9999 | |||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
50 pA | ||||
untrans vs shLuc | >0.9999 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | 0.9993 | |||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | 0.9995 | |||
shScr vs pSup | 0.9996 | |||
100 pA | ||||
untrans vs shLuc | 0.9997 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | 0.9923 | |||
shLuc vs shScr | 0.9995 | |||
shLuc vs pSup | 0.9978 | |||
shScr vs pSup | 0.9928 | |||
150 pA | ||||
untrans vs shLuc | 0.9909 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | 0.9964 | |||
shLuc vs shScr | 0.9959 | |||
shLuc vs pSup | >0.9999 | |||
shScr vs pSup | 0.9982 | |||
200 pA | ||||
untrans vs shLuc | 0.9818 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | 0.8433 | |||
shLuc vs shScr | 0.9915 | |||
shLuc vs pSup | 0.9674 | |||
shScr vs pSup | 0.8983 | |||
250 pA | ||||
untrans vs shLuc | 0.7984 | |||
untrans vs shScr | 0.9421 | |||
untrans vs pSup | 0.4089 | |||
shLuc vs shScr | 0.9945 | |||
shLuc vs pSup | 0.8912 | |||
shScr vs pSup | 0.8019 | |||
300 pA | ||||
untrans vs shLuc | 0.6045 | |||
untrans vs shScr | 0.8776 | |||
untrans vs pSup | 0.124 | |||
shLuc vs shScr | 0.9847 | |||
shLuc vs pSup | 0.714 | |||
shScr vs pSup | 0.5448 | |||
350 pA | ||||
untrans vs shLuc | 0.2035 | |||
untrans vs shScr | 0.8942 | |||
untrans vs pSup | 0.1894 | |||
shLuc vs shScr | 0.7588 | |||
shLuc vs pSup | 0.9865 | |||
shScr vs pSup | 0.6361 | |||
400 pA | ||||
untrans vs shLuc | 0.1775 | |||
untrans vs shScr | 0.7432 | |||
untrans vs pSup | 0.2523 | |||
shLuc vs shScr | 0.8662 | |||
shLuc vs pSup | 0.9987 | |||
shScr vs pSup | 0.844 | |||
450 pA | ||||
untrans vs shLuc | 0.0599 | |||
untrans vs shScr | 0.8942 | |||
untrans vs pSup | 0.2917 | |||
shLuc vs shScr | 0.4825 | |||
shLuc vs pSup | 0.9928 | |||
shScr vs pSup | 0.7592 | |||
500 pA | ||||
untrans vs shLuc | 0.0477 | |||
untrans vs shScr | 0.9921 | |||
untrans vs pSup | 0.6175 | |||
shLuc vs shScr | 0.2411 | |||
shLuc vs pSup | 0.8394 | |||
shScr vs pSup | 0.8383 | |||
550 pA | ||||
untrans vs shLuc | 0.0101 | |||
untrans vs shScr | 0.9971 | |||
untrans vs pSup | 0.8145 | |||
shLuc vs shScr | 0.0831 | |||
shLuc vs pSup | 0.4248 | |||
shScr vs pSup | 0.9297 | |||
600 pA | ||||
untrans vs shLuc | 0.0043 | |||
untrans vs shScr | >0.9999 | |||
untrans vs pSup | 0.68 | |||
shLuc vs shScr | 0.0299 | |||
shLuc vs pSup | 0.437 | |||
shScr vs pSup | 0.7694 | |||
650 pA | ||||
untrans vs shLuc | 0.0014 | |||
untrans vs shScr | 0.995 | |||
untrans vs pSup | 0.8638 | |||
shLuc vs shScr | 0.0266 | |||
shLuc vs pSup | 0.176 | |||
shScr vs pSup | 0.9617 | |||
700 pA | ||||
untrans vs shLuc | 0.0003 | |||
untrans vs shScr | 0.9998 | |||
untrans vs pSup | 0.9417 | |||
shLuc vs shScr | 0.0061 | |||
shLuc vs pSup | 0.0571 | |||
shScr vs pSup | 0.9735 | |||
750 pA | ||||
untrans vs shLuc | 0.0002 | |||
untrans vs shScr | 0.7664 | |||
untrans vs pSup | 0.9643 | |||
shLuc vs shScr | 0.0001 | |||
shLuc vs pSup | 0.038 | |||
shScr vs pSup | 0.6549 | |||
800 pA | ||||
untrans vs shLuc | 0.0007 | |||
untrans vs shScr | 0.5283 | |||
untrans vs pSup | 0.9959 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.038 | |||
shScr vs pSup | 0.6058 | |||
850 pA | ||||
untrans vs shLuc | 0.0041 | |||
untrans vs shScr | 0.2197 | |||
untrans vs pSup | >0.9999 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.0601 | |||
shScr vs pSup | 0.4478 | |||
900 pA | ||||
untrans vs shLuc | 0.0005 | |||
untrans vs shScr | 0.4401 | |||
untrans vs pSup | >0.9999 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.0174 | |||
shScr vs pSup | 0.6624 | |||
950 pA | ||||
untrans vs shLuc | 0.0001 | |||
untrans vs shScr | 0.1954 | |||
untrans vs pSup | 0.9999 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.006 | |||
shScr vs pSup | 0.466 | |||
1000 pA | ||||
untrans vs shLuc | 0.0004 | |||
untrans vs shScr | 0.1282 | |||
untrans vs pSup | 0.9992 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.0108 | |||
shScr vs pSup | 0.4014 | |||
1050 pA | ||||
untrans vs shLuc | 0.0001 | |||
untrans vs shScr | 0.2644 | |||
untrans vs pSup | 0.9762 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.0025 | |||
shScr vs pSup | 0.728 | |||
1100 pA | ||||
untrans vs shLuc | 0.0003 | |||
untrans vs shScr | 0.2803 | |||
untrans vs pSup | 0.999 | |||
shLuc vs shScr | <0.0001 | |||
shLuc vs pSup | 0.0087 | |||
shScr vs pSup | 0.5944 | |||
Fig. 4B | Parametric | One-way ANOVA post hoc Tukey | Threshhold: | |
shLuc vs shScr | 0.0249 | |||
shLuc vs pSup | 0.0421 | |||
shLuc vs untrans | 0.0094 | |||
shScr vs pSup | 0.9958 | |||
shScr vs untrans | 0.9991 | |||
pSup vs untrans | 0.9837 | |||
Half width: | ||||
shLuc vs shScr | 0.0002 | |||
shLuc vs pSup | 0.0165 | |||
shLuc vs untrans | <0.0001 | |||
shScr vs pSup | 0.9231 | |||
shScr vs untrans | 0.9846 | |||
pSup vs untrans | 0.7637 | |||
Fig. 4C | Parametric | Two-way ANOVA post hoc Tukey | -80 mV | |
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | >0.9999 | |||
shLuc vs untrans | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
shScr vs untrans | >0.9999 | |||
pSup vs untrans | >0.9999 | |||
-70 mV | ||||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | 0.9998 | |||
shLuc vs untrans | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
shScr vs untrans | >0.9999 | |||
pSup vs untrans | >0.9999 | |||
-60 mV | ||||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | 0.9982 | |||
shLuc vs untrans | 0.9994 | |||
shScr vs pSup | 0.9995 | |||
shScr vs untrans | >0.9999 | |||
pSup vs untrans | 0.9999 | |||
−50 mV | ||||
shLuc vs shScr | 0.9996 | |||
shLuc vs pSup | 0.9956 | |||
shLuc vs untrans | 0.998 | |||
shScr vs pSup | 0.9992 | |||
shScr vs untrans | 0.9999 | |||
pSup vs untrans | 0.9999 | |||
−40 mV | ||||
shLuc vs shScr | 0.999 | |||
shLuc vs pSup | 0.2815 | |||
shLuc vs untrans | 0.9959 | |||
shScr vs pSup | 0.3548 | |||
shScr vs untrans | 0.9999 | |||
pSup vs untrans | 0.3237 | |||
−30 mV | ||||
shLuc vs shScr | 0.0008 | |||
shLuc vs pSup | 0.0272 | |||
shLuc vs untrans | 0.0755 | |||
shScr vs pSup | 0.7329 | |||
shScr vs untrans | 0.315 | |||
pSup vs untrans | 0.9295 | |||
−20 mV | ||||
shLuc vs shScr | 0.0007 | |||
shLuc vs pSup | 0.0009 | |||
shLuc vs untrans | 0.0235 | |||
shScr vs pSup | 0.9999 | |||
shScr vs untrans | 0.5293 | |||
pSup vs untrans | 0.5778 | |||
−10 mV | ||||
shLuc vs shScr | 0.0007 | |||
shLuc vs pSup | 0.0013 | |||
shLuc vs untrans | 0.0183 | |||
shScr vs pSup | 0.9983 | |||
shScr vs untrans | 0.5872 | |||
pSup vs untrans | 0.7019 | |||
0 mV | ||||
shLuc vs shScr | 0.001 | |||
shLuc vs pSup | 0.0014 | |||
shLuc vs untrans | 0.015 | |||
shScr vs pSup | 0.9998 | |||
shScr vs untrans | 0.7013 | |||
pSup vs untrans | 0.7536 | |||
10 mV | ||||
shLuc vs shScr | 0.0005 | |||
shLuc vs pSup | 0.0008 | |||
shLuc vs untrans | 0.0083 | |||
shScr vs pSup | 0.9996 | |||
shScr vs untrans | 0.6986 | |||
pSup vs untrans | 0.7654 | |||
20 mV | ||||
shLuc vs shScr | 0.0011 | |||
shLuc vs pSup | 0.0017 | |||
shLuc vs untrans | 0.0064 | |||
shScr vs pSup | 0.9994 | |||
shScr vs untrans | 0.856 | |||
pSup vs untrans | 0.9091 | |||
30 mV | ||||
shLuc vs shScr | 0.0015 | |||
shLuc vs pSup | 0.0017 | |||
shLuc vs untrans | 0.0042 | |||
shScr vs pSup | >0.9999 | |||
shScr vs untrans | 0.9547 | |||
pSup vs untrans | 0.9613 | |||
Fig. 4D | Parametric | Two-way ANOVA post hoc Tukey | −80 mV | |
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | >0.9999 | |||
shLuc vs untrans | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
shScr vs untrans | >0.9999 | |||
pSup vs untrans | >0.9999 | |||
−70 mV | ||||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | 0.9998 | |||
shLuc vs untrans | >0.9999 | |||
shScr vs pSup | >0.9999 | |||
shScr vs untrans | >0.9999 | |||
pSup vs untrans | >0.9999 | |||
−60 mV | ||||
shLuc vs shScr | >0.9999 | |||
shLuc vs pSup | 0.9982 | |||
shLuc vs untrans | 0.9994 | |||
shScr vs pSup | 0.9995 | |||
shScr vs untrans | >0.9999 | |||
pSup vs untrans | 0.9999 | |||
−50 mV | ||||
shLuc vs shScr | 0.9996 | |||
shLuc vs pSup | 0.9956 | |||
shLuc vs untrans | 0.998 | |||
shScr vs pSup | 0.9992 | |||
shScr vs untrans | 0.9999 | |||
pSup vs untrans | 0.9999 | |||
−40 mV | ||||
shLuc vs shScr | 0.999 | |||
shLuc vs pSup | 0.2815 | |||
shLuc vs untrans | 0.9959 | |||
shScr vs pSup | 0.3548 | |||
shScr vs untrans | 0.9999 | |||
pSup vs untrans | 0.3237 | |||
−30 mV | ||||
shLuc vs shScr | 0.0008 | |||
shLuc vs pSup | 0.0272 | |||
shLuc vs untrans | 0.0755 | |||
shScr vs pSup | 0.7329 | |||
shScr vs untrans | 0.315 | |||
pSup vs untrans | 0.9295 | |||
−20 mV | ||||
shLuc vs shScr | 0.0007 | |||
shLuc vs pSup | 0.0009 | |||
shLuc vs untrans | 0.0235 | |||
shScr vs pSup | 0.9999 | |||
shScr vs untrans | 0.5293 | |||
pSup vs untrans | 0.5778 | |||
−10 mV | ||||
shLuc vs shScr | 0.0007 | |||
shLuc vs pSup | 0.0013 | |||
shLuc vs untrans | 0.0183 | |||
shScr vs pSup | 0.9983 | |||
shScr vs untrans | 0.5872 | |||
pSup vs untrans | 0.7019 | |||
0 mV | ||||
shLuc vs shScr | 0.001 | |||
shLuc vs pSup | 0.0014 | |||
shLuc vs untrans | 0.015 | |||
shScr vs pSup | 0.9998 | |||
shScr vs untrans | 0.7013 | |||
pSup vs untrans | 0.7536 | |||
10 mV | ||||
shLuc vs shScr | 0.0005 | |||
shLuc vs pSup | 0.0008 | |||
shLuc vs untrans | 0.0083 | |||
shScr vs pSup | 0.9996 | |||
shScr vs untrans | 0.6986 | |||
pSup vs untrans | 0.7654 | |||
20 mV | ||||
shLuc vs shScr | 0.0011 | |||
shLuc vs pSup | 0.0017 | |||
shLuc vs untrans | 0.0064 | |||
shScr vs pSup | 0.9994 | |||
shScr vs untrans | 0.856 | |||
pSup vs untrans | 0.9091 | |||
30 mV | ||||
shLuc vs shScr | 0.0015 | |||
shLuc vs pSup | 0.0017 | |||
shLuc vs untrans | 0.0042 | |||
shScr vs pSup | >0.9999 | |||
shScr vs untrans | 0.9547 | |||
pSup vs untrans | 0.9613 | |||
Fig. 5A | Parametric | Student’s t test | Ctrl vs CPT | <0.00001 |
Fig. 5B | Parametric | One-way ANOVA post hoc Tukey | shLuc vs shScr | 0.8937 |
shLuc vs pSup | 0.885 | |||
shScr vs pSup | 0.9996 |