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. 2017 Nov;92(5):588–600. doi: 10.1124/mol.117.108555

Fig. 2.

Fig. 2.

Effects of PTX on U50,488H-induced KOPR phosphorylation. Cells were pretreated with 200 ng/ml PTX for 2 hours followed by a 2-minute incubation with 3 µM U50,488H. Experiments were performed as described in the Fig. 1 legend. The data shown are reported as the mean ± S.E.M. (n = 3) and were analyzed by two-way ANOVA followed by Bonferroni post hoc test (***P < 0.001, compared with control (ctrl) group; ###P < 0.001, compared with U50,488H-treated group).