Table 1.
Strain | IPE5-4 | IPE5-4-UD-4 | |
---|---|---|---|
Fermentation process | Shake flask | Shake flask | Fermenter |
Substrate consumption | |||
Substrate concentration (g L−1) | 99.92 ± 0.91 | 101.83 ± 3.81 | 120.94 ± 4.02 |
Substrate consumption time (h) | 72 | 72 | 48 |
Concentration of residual substrate (g L−1) | 52.98 ± 1.93 | 52.28 ± 2.19 | 35.38 ± 2.34 |
Consumption rate of substrate (g L−1 h−1)a | 0.65 | 0.69 | 1.78 |
Acetoin production | |||
Maximum concentration (g L−1) | 11.45 ± 0.11 | 17.20 ± 0.19 | 28.83 ± 0.65 |
Acetoin yield (g g−1)b | 0.24 | 0.35 | 0.34 |
Acetoin productivity (g L−1 h−1)c | 0.16 | 0.24 | 0.60 |
CAcetoin/CdGlucose | 0.33 ± 0.01 | 0.47 ± 0.03 | 0.46 ± 0.02 |
aConsumption rate of substrate was calculated by [initial concentration of specific substrate (g L−1) − residual concentration of specific substrate (g L−1)]/substrate consumption time (h)
bAcetoin yield was calculated by g acetoin/g consumed substrate
cAcetoin productivity was calculated by maximum concentration of acetoin (g L−1)/substrate consumption time (h)
d C Acetoin meant the molar concentration of carbon in acetoin, and C Glucose meant the molar concentration of carbon in glucose