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. 2017 Oct 10;18:765. doi: 10.1186/s12864-017-4157-9

Table 2.

Quantitation of biofilm architecture

Items 2 days 4 days 6 days
Total biomass (μm3/μm2) 19.86 ± 3.11b 24.84 ± 1.60a 19.67 ± 2.14b
Maximum thickness (μm) 9.20 ± 1.10b 13.20 ± 1.10a 8.40 ± 0.89b
Average thickness (μm) 7.00 ± 1.51ab 8.42 ± 1.55a 6.20 ± 1.38b

*Quantitation of biofilm parameters, including total biomass, maximum thickness, average thickness, were evaluated using COMSTAT. The results are means of datasets obtained from analysis of five CLSM images acquired at random positions in each of the biofilms. Standard deviations (SD) are also shown at the end of each of the means. Different superscript letters denote significant differences within a row (Tukey’s HSD: P < 0.05)