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. 2017 Oct 10;10:471. doi: 10.1186/s13071-017-2415-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Results of tests for isolation by distance showing the linear regression of genetic differentiation F_ST/(1-F_ST) by geographical distance (km) (a), and temporal distance (generations) (b), with the best line of linear fit and R² values displayed by region. Tests for isolation by distance were completed using Rousset’s procedure [63] implemented in the “isolation by distance” v3.23 web service [64]. Geographical distances were generated using the web-based “geographic matrix generator” v1.2.3 [65], and the significance of the regression (P) and strength of the correlation (R ²) were tested by a Mantel test with 10,000 randomizations [66]