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. 2017 Oct 10;10:231. doi: 10.1186/s13068-017-0919-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The pNsAtWRI1 vector and identification of the plasmid in the transformants. a Schematic map of the pNsAtWRI1 plasmid. b Detection of plasmids in NsAtWRI1 and WT. Genomic sequences of AtWRI1 (1.3 kb) and 18S rDNA (380 bp) were PCR amplified and the products were electrophoresed on agarose gels. c Western blotting of FLAG-tagged NsAtWRI1. The expected size of FLAG-tagged AtWRI1 was 49.4 kD, but running around 55 kD. AtpB (the CF₁ ß subunit of ATP synthase of expected size 72.6 kD) was used as a loading control. d Relative intensity of AtWRI1-FLAG protein, which was calculated as the ratio of AtWRI1-FLAG vs AtpB. WT wild type, N normal conditions, NL nitrogen limitation, O osmotic stress