TABLE 1 .
Measured cellular quotas for TPP, TMP, and ThF in E. huxleyi CCMP2090a
| Treatment | Initial concn (nmol liter−1) |
Growth rate (day−1) |
Mean quota per cell (pmol cell−1) |
T sum/C (nmol g C−1) |
|||
|---|---|---|---|---|---|---|---|
| TPP | TMP | ThF | T sum | ||||
| Replete conditions | |||||||
| Luxury thiamin | 300 | 0.74 (0.05) | 4.47 × 10−7
(3.66 × 10−7) |
4.08 × 10−7
(3.62 × 10−8) |
2.6 × 10−7
(1.48 × 10−7) |
11.15 × 10−7
(1.83 × 10−7) |
327.7 (62.5) |
| Replete thiamin | 10 | 0.71 (0.04) | 1.48 × 10−7
(4.00 × 10−8) |
5.78 × 10−8
(3.49 × 10−8) |
2.11 × 10−8
(1.05 × 10−8) |
2.27 × 10−7
(2.48 × 10−8) |
54.3 (4.7) |
| Replete HMP | 10 | 0.73 (0.06) | 1.43 × 10−7
(8.80 × 10−8) |
1.42 × 10−7
(8.00 × 10−8) |
1.94 × 10−8
(7.10 × 10−9) |
3.04 × 10−7
(7.32 × 10−8) |
79.5 (22.5) |
| Limiting conditions | |||||||
| Limiting thiamin | 0.5 | 0.25 (0.13) | 1.60 × 10−7
(6.78 × 10−9) |
2.89 × 10−8
(1.48 × 10−8) |
3.31 × 10−8
(2.26 × 10−8) |
2.22 × 10−7
(7.28 × 10−9) |
31.6 (3.4) |
| Limiting HMP | 0.1 | 0.21 (0.11) | 1.14 × 10−7
(7.12 × 10−9) |
2.08 × 10−8
(1.97 × 10−9) |
2.30 × 10−8
(6.00 × 10−9) |
1.58 × 10−7
(1.36 × 10−8) |
28.9 (3.0) |
a
Numbers in parentheses represent the standard deviation from biological triplicates. C, carbon; T sum, the sum of the three thiamin states quantified (TPP, TMP, and ThF).