Figure 7.

(A) ³H-dopamine uptake in HEK-hVMAT2 cells following exposure to PFOS caused a dose-dependent inhibition of dopamine transport through VMAT2. Fractions of HEK-hVMAT2 cells were incubated with PFOS or DMSO followed by incubation with ³H-dopamine. Resultant radioactive uptake into vesicles is shown. Results demonstrate PFOS inhibits dopamine uptake by VMAT2 with an IC₅₀ = 4.56 Μm; (B) Reduction in dopamine uptake by VMAT2 was not due to PFOS-induced cytotoxicity of HEK-hVMAT2 cells, as assessed by LDH release. HEK-hVMAT2 cells were incubated for 10 min in increasing concentrations of PFOS at similar concentrations used for uptake. Each experiment was performed in triplicate.