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. Author manuscript; available in PMC: 2018 Jul 25.
Published in final edited form as: Cell Rep. 2017 Jul 25;20(4):785–793. doi: 10.1016/j.celrep.2017.06.076

Figure 5. The effect of CRISPR-derived activators on Kit mRNA expression.

Figure 5

(A) Shows that no mRNA is induced in the absence of 5-AZA-C. Control stands for myeloid cells expressing the CRISPR activator protein (CAS9-SAM) but no guide RNA. Sg1, Sg2, and Sg3 refer to different guides directed to three different locations in the promoter. The significance of the increase in Kit mRNA levels, Plus 5-AZA-C, in Sg1 over control: p < 0.011, in Sg2 over control: p < 0.028, in Sg3 over control: p < 0.02. (B) And (C) are ChIP-qPCR experiments probing for H3K27me3 along the Kit promoter in the absence of any guide RNA (gray line), and the bluish line guide 3.