Fig. S4.

Bilateral administration of AAV2-CMV-PI-Cre-rBG into the MPOA of adult Prlr^lox/lox female mice did not induce GFP expression or restrict pSTAT5 expression in adjacent regions, including the PVN (A) or ARN (B), showing the localized deletion of the Prlr gene using this approach. Note that a few neurons in the ARN express GFP, suggesting that there may have been limited retrograde infection of neurons that project from the arcuate to the MPOA. (C) Reproductive data from the MPOA-specific Prlr KO animals (Prlr^lox/lox/AAV-Cre). There was no effect on time to mating (n = 5–7) and duration of pregnancy in these animals (n = 5–7).