Fig. S2.

Enhanced Th2 responses but normal recruitment and expansion of T cell populations in mb1^creIL-4Rα^–/lox mice infected with L. major LV39. (A–C) mb1^creIL-4Rα^–/lox BALB/c and control mice were infected s.c. with 2 × 10⁶ L. major LV39 promastigotes into the hind footpad. At week 8 postinfection, total LN cells were restimulated with anti-CD3 or SLA for 72 h, and cell supernatants were analyzed for the production of IL-4 (A), IL-13 (B), and IFN-γ (C). (D–G) mb1^creIL-4Rα^–/lox BALB/c and control mice were infected s.c. with 2 × 10⁶ L. major LV39 promastigotes into the hind footpad. Draining LN cells were FACS-stained and gated (D) for recruitment of CD3⁺CD4⁺ Th cells (E), expansion of effector memory CD4⁺ T cells (CD3⁺CD4⁺CD44^hiCD62L^lo) (F), and central memory CD4⁺ T cells (CD3⁺CD4⁺CD44^hiCD62L^hi) (G). A representative of two individual experiments is shown with mean values ± SEM. Statistical analysis was performed defining differences to littermate IL-4Rα^–/lox BALB/c control mice as significant (*P ≤ 0.05, **P ≤ 0.01).