Fig. S7.
Infection kinetics in BALB/c, IL-4Rα−/−, and IL-4−/−/IL-4Rα−/− mice to generate L. major LV39-primed B cells. (A and B) Mice were infected s.c. with 2 × 106 promastigote L. major LV39 parasites into the hind footpad to determine footpad swelling (A) and parasite burdens in homogenized footpads and in draining LNs (B) at week 8 after infection (n = 11 mice per group). (C–E) Total LN cells were restimulated with anti-CD3 or SLA for 72 h, and cell supernatants were analyzed for the production of IL-4 (C), IL-13 (D), and IFN-γ (E) by ELISA. (F and G) Antigen-specific IgG1 (F) and IgG2a (G) antibody isotypes were quantified from infected sera by ELISA. A representative of two individual experiments is shown with mean values ± SEM. Statistical analysis was performed defining differences to WT BALB/c control mice as significant (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).