Fig. S9.

Successful reconstitution of chimeric mice with donor BM cells following irradiation. Irradiated µMT mice were reconstituted with 50% µMT BM and 50% WT BM (B-WT chimeras), 50% µMT BM and 50% IL-4^−/− BM (B-IL-4^−/−), and 50% µMT BM. As controls, µMT mice were reconstituted with 100% µMT BM (µMT), 100% IL-4^−/− BM (IL-4^−/−), and C57BL/6 recipient mice with 100% C57BL/6 BM (BL/6). At 8 wk postreconstitution, mouse blood was collected and leukocytes FACS-stained for total numbers of CD3⁺CD4⁺ Th cells (A), CD19⁺B220⁺ B cells (B), CD4⁻CD11c^hi dendritic-like cells (C), and CD11b^hiCD11c⁻CD3⁻ macrophage-like cells (D). Data are representative of two independent experiments (n = 6/7 mice/group). Statistical analysis was performed defining differences to B-WT mice as significant (****P ≤ 0.0001).