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. 2017 Sep 18;114(40):10731–10736. doi: 10.1073/pnas.1708264114

Fig. S4.

Fig. S4.

Expression of intracellular Bcl2 in CLL cases with high or low ROR1 expression by flow cytometry. In A and B, representative histograms show the fluorescence of cells labeled with PE-conjugated anti-Bcl2 mAb (shaded histograms) or PE-conjugated nonspecific IgG of the same isotype (black solid line open histograms). The negative control (Raji cells) is shown in A. The positive control (Jurkat cells) is shown in B. C shows representative CLL cases from cohort A with high (shaded histogram) or low (gray solid line histogram) levels of ROR1 expression. D shows the relative expression of intracellular Bcl2 in representative CLL cases with high (purple and orange solid lines histograms) or low (dark and light green solid lines histograms) expression of ROR1 and healthy individuals (blue and red solid lines histograms). In E, a graphical representation of the data showed in A–D. The bar graphs show the fluorescence of cells labeled with PE-conjugated anti-Bcl2 mAb. Iso (PE-conjugated nonspecific IgG isotype), Raji cells (negative control), Jurkat cells (positive control), Healthy cases (n = 6), and CLL cases with low or high levels of ROR1 expression (n = 4 of each). Error bars represent the SD.