Fig. 6.

Monocolonization of GF mice with MS-associated bacteria mediates T lymphocyte differentiation. (A–C) Representative flow cytometry plots (A) and quantification (B and C) of CD4⁺IFNγ⁺ lymphocytes (B) and CD4⁺IL-10⁺ lymphocytes (C) within the live cell population in GF mice colonized with A. calcoaceticus, A. muciniphila, and P. distasonis. GF mice and specific pathogen-free (SPF) mice are used as controls. n = 3–8 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA with Tukey adjustment for multiple comparisons. Data are shown as mean ± SEM. CLN, cervical lymph nodes; MLN, mesenteric lymph nodes.