Table 8.
Hsp26 and Hsp42 are dispensable for [PSI+] propagation
Donor arg82Δ pARG82 [PSI+x] | Recipient strain no. (ρo p1520 pRS313) | %Ura+ cytoductants (total) |
5548 [PSI+1] | WT, 4812 | 88 (25) |
5549 [PSI+2] | WT, 4812 | 86 (14) |
5548 [PSI+1] | ipk1Δ kcs1Δ, 5536 | 0 (15) |
5549 [PSI+2] | ipk1Δ kcs1Δ, 5536 | 0 (22) |
5548 [PSI+1] | hsp26Δ, 5558 | 98 (40) |
5549 [PSI+2] | hsp26Δ, 5558 | 100 (23) |
5548 [PSI+1] | hsp42Δ, 5559 | 100 (25) |
5549 [PSI+2] | hsp42Δ, 5559 | 90 (21) |
Cytoduction donors were ρ+, and recipients were ρo. Cytoductants were identified as those with recipient nuclear genotype and donor cytoplasm (ρ+). The recipients carried p1520 bearing the ura3-14 allele, enabling scoring of [PSI+] as Ura+.