Figure 3.
Distribution of r Mutation in Ilaria_F2 Segregating Population and in F. vesca Germplasm.
(A) Allelic status of FveGA20ox4 in the Ilaria_F2 segregating population. The 154 plants were scored as runnering [R] or runnerless [r] when the number of stolons was >3 or ≤3, respectively. The FveGA20ox4 wild-type allele confers [R] phenotype when present in simple or double dose. The ΔFvega20ox4 mutant allele confers [r] phenotype when presents in double dose. Error bars show se.
(B) Genotyping of FveGA20ox4 and phenotyping of runnering. FveGA20ox4 is the wild-type gene, and ΔFvega20ox4 is the deleted mutant version. Circle, homozygous FveGA20ox4/FveGA20ox4; triangle, homozygous ΔFvega20ox4/ΔFvega20ox4. [R], runnering phenotype; [r], runnerless phenotype.
(C) Genetic structure of 25 F. vesca accessions. Genetic structure was determined by examining 37 microsatellite loci distributed throughout the genome. Each accession is represented by a single vertical line, which is partitioned into gray and black segments in proportion to the estimated membership in the two clusters (K = 2).