Figure 4.

FveGA20ox4 Transcripts Are Localized in the Axillary Meristem and Stolon.

(A) Tissue sampling of PC (in red circle), leaf (L), and stolon (ST; blue arrow). PC includes stem (S), SAM, and AXMs. R, roots.

(B) Relative expression of FveGA20ox4 in leaf, PC, and stolon of ‘Fb2’ wild type and ‘RdV’ r mutant. Normalization with FveActin. n = 3 ± se.

(C) to (H) In situ hybridization of FveGA20ox4 on the ‘Fb2’ tfl1 SAM ([C] and [D]), AXM ([E] and [F]), and meristem of the stolon during elongation ([G] and [H]). Upper panel of (C), (E), and (G), antisense probe; lower panel of (D), (F), and (H), sense probe. Samples were processed side-by-side with sense and antisense probes. MD, meristematic dome; LT, leaflet; LP, leaf primordium; ST, stolon; StM, stolon meristem. Bar = 0.1 mm.