Figure 6.
Deletion Leads to a Loss of FveGA20ox4 Catalytic Activity.
(A) Simplified GA biosynthetic pathway. Intermediates (blue), bioactive (green), and inactive (red) GAs are represented for the last steps of the pathway. Enzymes are framed and colored according to the product of the reaction.
(B) and (C) HPLC-radiochromatograms of products from incubations of recombinant FveGA20ox4 wild type (B) and ΔFvega20ox4 mutant (C) proteins with 14C-labeled GA12. Identities of substrate and product were confirmed as methyl esters trimethylsilyl ethers by gas chromatography-mass spectrometry by comparison of their mass spectra with published spectra.