Table 1. Natural Variation Analysis of R927 and the Four Polymorphic Sites (3, 4, 5, and 6) in the Sw-5 LRR Domain of Wild Tomato Species.
| Accessions | Taxon | HRa | ELISAb | R927 | 3 (I) | 4 (FE) | 5 (MNLPYYDIL) | 6 (VG) |
|---|---|---|---|---|---|---|---|---|
| LA0370 | S. peruvianum | + | − | √ | √ | √ | √ | √ |
| LA1407 | S. cheesmaniae | + | − | √ | √ | √ | √ | √ |
| LA1722 | S. corneliomulleri | + | − | √ | √ | √ | √ | √ |
| LA1365 | S. huaylasense | + | − | √ | √ | √ | √ | √ |
| LA2809 | S. huaylasense | + | − | √ | √ | √ | √ | √ |
| LA1350 | S. arcanum | + | − | √ | √ | √ (FK) | √ | √ |
| LA0103 | S. corneliomulleri | − | − | √ | S | √ (FK) | √ | √ |
| LA2581 | S. peruvianum | − | + | √ | S | √ (FK) | √ | √ |
| LA4445 | S. peruvianum | − | − | √ | SM | √ (FK) | √ | √ |
| LA0716 | S. pennellii | − | n | √ | √ | YK | KNFPYYDIV | VE |
| LA0751 | S. pennellii | − | + | √ | √ | YK | KNFPYYDIV | VE |
| LA0927 | S. cheesmaniae | − | n | √ | T | DK | KNFP-YGIG | IQ |
| LA0747 | S. galapagense | − | + | √ | T | DK | KNFP-YGIG | IQ |
| LA1204 | S. lycopersicum | − | + | √ | T | DK | KNFP-YGIG | IQ |
| LA0446 | S. peruvianum | − | + | √ | T | DK | KNFP-YGIG | IQ |
| LA0411 | S. pimpinellifolium | − | + | √ | T | DK | KNFP-YGIG | IQ |
| LA1331 | S. corneliomulleri | − | + | √ | N | DK | KNFP-YNIG | IG |
| LA2113 | S. neorickii | − | n | √ | T | DK | KNLP-YGIV | IQ |
| LA1777 | S. habrochaites | − | + | √ | T | DK | KNLP-YGTG | IG |
| LA1358 | S. huaylasense | − | − | √ | T | DE | KNYP-YRMG | IG |
| LA1930 | S.chilense | − | + | √ | N | DK | KNFP-YNIGL | IG |
| LA0445 | S. peruvianum | − | − | √ | √ | YK | KNFPYYDIV | √ |
| LA1028 | S. chmielewskii | − | − | Truncated | ||||
| LA2951 | S. lycopersicoides | − | + | Truncated | ||||
| LA1974 | S. sitiens | − | n | No PCR product |
Twenty-seven accessions representing all 17 wild tomato species from South America were obtained from TGRC, while two accessions of tomato seeds, S. ochranthum LA2160 and S. juglandifolium LA3322, did not germinate and were not used in further analysis. The “√” refers to the conserved site. As Sw-5b with the FK site variation is still functionally resistant to TSWV, we still mark this site as a functionally conserved variation. The “n” represents “not tested.”
a
Sw-5b homolog is coexpressed with NSm for the HR assay.
b
The presence of virus in the systemic leaves of wild tomato species inoculated with TSWV was assayed by ELISA.