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. Author manuscript; available in PMC: 2017 Oct 11.
Published in final edited form as: ACS Biomater Sci Eng. 2015 Dec 10;2(1):131–140. doi: 10.1021/acsbiomaterials.5b00446

Figure 2.

Figure 2

Characterization of decellularized fetal and adult brain ECM. (a) DAPI images taken with an inverted fluorescence microscope (Keyence, BZ-X700 series, Itasca, IL) indicating the effective removal of cells from the fetal and adult porcine brains. Little to no presence of cell nuclei seen in the decellularized samples in comparison to a partially decellularized sample. Scale bars: 100μm (b) DNA content in the decellularized brain tissues versus adult and fetal whole brains quantified by Pico green assay. DNA content was found to be less than 30ng/mg dry weight, close to the values found in commercially available matrigel. (c) Collagen and sGAG content of the fetal and adult porcine brain ECM. The overall collagen-sGAG content was measured to be higher for fetal ECM, although the ratio of sGAG to collagen was greater for adult brain ECM. (d) Protein gel showing A, B, C, D corresponding to Pepsin, Marker, Fetal and Adult ECM, respectively. Multiple bands in the high molecular weight range of the marker, indicative of the complexity of brain ECM with many peptides.