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. 2017 Aug 14;102(5):1261–1269. doi: 10.1189/jlb.4A0317-116R

Figure 3. MR1 is essential for induction of B cell antibody production by MAIT/THP-1/BL21 supernatant.

Figure 3.

We cultured B cells from different subjects for 7 d in supernatant from subject-matched MAIT cells and THP-1 cells incubated overnight with BL21. (A–C) IgA, IgG, and IgM levels for the different conditions, with anti-MR1 (10 µg/ml), anti-IL-12/18, or both at 5 µg/ml each during initial MAIT/THP-1/BL21 stimulation or anti-CD40L (20 µg/ml) added to supernatant after MAIT culture and before addition to B cells. Data are means ± sem, and significance was determined by 1-way ANOVA using Tukey’s multiple-comparisons test. Data are representative of 2 independent experiments. *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.00001.