Table 1. Primer sequences and volumes use in the multiplex PCR assay.

All primers were used with a concentration of 10 μM.

Primer name	Primer sequence	Volume [μl]	References
VF2_tl	5’-TGTAAAACGACGGCCAGTCAACCAACCACAAAGACATTGGCAC-3’	1.5	Ward et al. (2005)
FishR1_tl	5’-CAGGAAACAGCTATGACACTTCAGGGTGACCGAAGAATCAGAA-3’	0.75	Ward et al. (2005)
FishR2_tl	5’-CAGGAAACAGCTATGACACCTCAGGGTGTCCGAARAAYCARAA-3’	0.75	Ward et al. (2005)
Shark150R	5’-AAGATTACAAAAGCGTGGGC-3’	0.375	Fields et al. (2015)
Shark474F	5’-CHATTTCCCAATATCAAACACC-3’	0.1875	This study