Table 3. Five possible amplification options of the multiplex PCR assay showing the different amplicons and percentage of the samples from Guangzhou, China (n = 200) that amplified for each option.

COI	Shark474	Shark150	% of samples
+	+	+	0.5%
-	+	+	37.0%
-	+	-	7.0%
-	-	+	40.5%
-	-	-	15%

+ denotes positive amplification, - indicates no amplification.