Fig 5. LLC metastasis growth in DUSP3^-/- mice is associated with the presence of higher monocytes and macrophages in lungs and with increased proliferation.

Immune cell population phenotyping in LLC tumour-bearing lungs by flow cytometry. (A) Percentages of neutrophils, eosinophils, alveolar macrophages and monocytes/macrophages in DUSP3^+/+ and DUSP3^-/- lungs. (B) Absolute number of neutrophils, eosinophils, alveolar macrophages and monocytes/macrophages in DUSP3^+/+ and DUSP3^-/- lungs. (C-E) Monocytes/macrophages phenotype characterization in LLC tumours: Comparison (C) and percentage (D) of Ly6B^hi, Ly6B^int and Ly6B^low macrophages in DUSP3^+/+ and DUSP3^-/- mice. (E) Expression of specific macrophages markers in distinct Ly6B⁺ populations. Alveolar Ф = alveolar macrophages; Mo/MФ = monocytes/macrophages. Student-t-test was used for statistical analysis. *p < 0,05. 3 mice were used in each group and for each experiment. Data shown are representative of 4 different experiments.