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| Gene | Primer sequence | Amplicon size |
|---|---|---|
| Tlh | F: AGAACTTCATCTTGATGACACTGC | 401 bp |
| R: GCTACTTTCTAGCATTTTCTCTGC | ||
| Trh | F: CATAACAAACATATGCCCATTTCCG | 500 bp |
| R: TTGGCTTCGATATTTTCAGTATCT | ||
| Tdh | F: GTAAAGGTCTCTGACTTTTGGAC | 269 bp |
| R: TGGAATAGAACCTTCATCTTCACC |
Primer sequences used in the MPN PCR assay for identification of tlh, trh, and tdh.
