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. 2017 Oct;58(12):5164–5176. doi: 10.1167/iovs.16-20736

Figure 8.

Figure 8

Time- and tissue compartment–dependent changes in mRNA expression of key cytokines in WT mice. Mouse eyes were subjected to 10% DC subthreshold micropulse laser and harvested at indicated time points. Eyes were dissected, mRNA was extracted separately from NSR and RPE-choroid, and real-time PCR quantification was performed. All reactions were performed in duplicate. The relative amounts of mRNAs were calculated using the 2−ΔΔCq method, using Actb as endogenous control. (A) Cxcl2. (B) Cxcr4. (C) Il1b. (D) Il6. (E) Ifng. (F) Tnfa. Data are means ± SEM; #P < 0.05 versus control; *P < 0.05 between NSR and RPE-choroid; n = 5 mice per time point.