Fig. 4.

Transfection efficacy of the optimal PBAE (536 25 w/w) and positive control (PEI 25kDa 2 w/w) NP formulations for all cell lines. Transfection of eGFP DNA with 536 25 w/w NPs was effective and consistent to all cancer cells. In addition, the eGFP expression in all HCC lines was also specific over healthy hepatocytes. While also cancer-selective, PEI 25kDa 2 w/w NPs did not promote consistently high eGFP expression to all HCC cells. A. EGFP expression of all cell lines 48 hours after transfection with 536 25 w/w (left) or PEI 25kDa 2 w/w (right). B. Flow cytometry gating and microscopy images of two representatives of HCC lines and THLE-3 hepatocytes treated with 536 25 w/w (left column) or PEI 25kDa 2 w/w (right column). * P < 0.01